Advantages and disadvantages of the serial dilution agar plate technique

#Advantages and disadvantages of the serial dilution agar plate technique series
#Advantages and disadvantages of the serial dilution agar plate technique free

During spreading hold the plate lid with the base of your thumb and index finger and use the tip of your thumb and middle finger to rotate the base.

Then touch the rod to the agar surface away from the inoculum to cool it.

After farming the glass rod, lift the lid of the plate and use it as a shield from airborne contamination.

Sterilize the L-shaped glass spreader by dipping this into 90% alcohol and then flame the glass spreader.

Properly dispose of the pipetting instrument used to inoculate the medium, because it is contaminated.

#Advantages and disadvantages of the serial dilution agar plate technique series

Take a clean and sterile Pipette and Pipette out 0.1 ml sample from the appropriate desired dilution series onto the center of the surface of an agar plate.

Lift the plateвЂ™s lid and use it as a shield to protect it from airborne contamination.

Mention the organism name, type of agar, date, and the platerвЂ™s name or initials.

Label the nutrient agar plate with Wax marking pencil.

Prepare different dilutions of the sample.

#Advantages and disadvantages of the serial dilution agar plate technique free

The main principle behind this Spread Plate technique is that as the Petri dish rotated, at some stage, single cells will be deposited with the bent glass rod on to the agar surface, these cells will be separated from each other by a distance sufficient to allow the colonies that develop to be free from each other. With an accurately diluted sample, cells (CFUs) will be deposited far enough individual on the agar surface to grow into individual colonies. The spread plate technique is a method of isolation in which a diluted microbial sample containing more than one microorganism is deposited on a solidified agar plate and spread uniformly across the surface with an L-shaped glass rod while the media plate is spun on a turntable.

The main purpose of the spread plate method is to isolate or count the individual bacteria present in a diluted sample containing a mixture of different species. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory.ДЄn ideal spread plate should contain visible and isolated colonies of bacteria that are evenly distributed in the plate and are countable. The spread plate method is used to isolate individual colonies from a diluted sample of mix population. 9 References Spread Plate Method in microbiology